Four zones are clearly distinguishable, starting from the inner advancing front of the lesion. These are the (1) translucent zone, (2) dark zone, (3) body of the lesion and (4) surface layer.

Zones of enamecarieTranslucenzone {TZ)

First recognizable zone of alteration

Advancing front of the lesion

Half the lesions demonstrate TZ, not always present

Seen in longitudinal ground sections in clearing (quinoline – RI – 1.62)

TZ appears structureless

Pore volume – I% (compared to 0.1 % of sound enamel)

Dark zone

Lies adjacent and superficial to the translucent zone Positive zone

Shows positive birefringence (in contrast to sound enamel.

Pore volume of 2-4% (polarized light)Presence of small pores; large molecules of quinoline are unable to penetrateMicropore system – gets filled with air and becomes dark

Medium like water may penetrate